pEF-ManII-GFP
(Plasmid #160905)

• Purpose: Expresses the Golgi marker ManII-GFP in mammalian cells
• Depositing Lab: Benjamin Glick
• Publication: Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591. doi: 10.1091/mbc.E20-09-0591.

Backbone

• Vector backbone: pEF/myc/ER
• Backbone size w/o insert (bp): 5300
• Total vector size (bp): 9600
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MAN2A1
• Species: H. sapiens (human)
• Insert Size (bp): 4176
• GenBank ID: 4124
• Entrez Gene: MAN2A1 (a.k.a. AMan II, GOLIM7, MANA2, MANII)
• Promoter: EF-1a
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GGCACTTGATGTAATTCTCCTTGGAATT
• 3′ sequencing primer: cctgctattgtcttcccaatcctcc

Resource Information

• Supplemental Documents:
  • pEF-ManII-GFP Snapgene file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry