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Addgene

pT-58
(Plasmid #16088)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16088 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEX-KG
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    UBA2
  • Alt name
    SUMO activating enzyme (E1)
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1912
  • Entrez Gene
    UBA2 (a.k.a. YDR390C, UAL1)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT-58 was a gift from Yoshiko Kikuchi (Addgene plasmid # 16088 ; http://n2t.net/addgene:16088 ; RRID:Addgene_16088)
  • For your References section:

    Comparative analysis of yeast PIAS-type SUMO ligases in vivo and in vitro. Takahashi Y, Toh-E A, Kikuchi Y. J Biochem (Tokyo). 2003 Apr . 133(4):415-22. 10.1093/jb/mvg054 PubMed 12761287