pCDF-NtXR1AtR2B2NtS1
(Plasmid
#160879)
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PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana Rubisco chaperone proteins raf2, and bsd2 in E. coli.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 160879 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDF-1b
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namerbcS-S1, rbcX, raf1, raf2, bsd2
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SpeciesA. thaliana (mustard weed); Nicotiana tabacum
- Promoter rbcS-S1: pBAD, chaperones: T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDF-NtXR1AtR2B2NtS1 was a gift from Maureen Hanson (Addgene plasmid # 160879 ; http://n2t.net/addgene:160879 ; RRID:Addgene_160879) -
For your References section:
Small subunits can determine enzyme kinetics of tobacco Rubisco expressed in Escherichia coli. Lin MT, Stone WD, Chaudhari V, Hanson MR. Nat Plants. 2020 Oct;6(10):1289-1299. doi: 10.1038/s41477-020-00761-5. Epub 2020 Sep 14. 10.1038/s41477-020-00761-5 PubMed 32929197