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Addgene

pET-NtLNtC60αESC60β
(Plasmid #160875)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160875 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-11a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rbcL, cpn60α, cpn60β, groES
  • Species
    Nicotiana tabacum, Escherichia coli
  • Promoter rbcL: pBAD, chaperones: T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-NtLNtC60αESC60β was a gift from Maureen Hanson (Addgene plasmid # 160875 ; http://n2t.net/addgene:160875 ; RRID:Addgene_160875)

  • For your References section:

    Small subunits can determine enzyme kinetics of tobacco Rubisco expressed in Escherichia coli. Lin MT, Stone WD, Chaudhari V, Hanson MR. Nat Plants. 2020 Oct;6(10):1289-1299. doi: 10.1038/s41477-020-00761-5. Epub 2020 Sep 14. 10.1038/s41477-020-00761-5 PubMed 32929197
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