pT-77
(Plasmid #16087)

• Depositing Lab: Yoshiko Kikuchi
• Publication: Takahashi et al J Biochem (Tokyo). 2003 Apr . 133(4):415-22.

Backbone

• Vector backbone: pET21b
• Backbone size w/o insert (bp): 5443
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ull1 delta440
• Alt name: SUMO ligase (E3)
• Alt name: Ull1
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1395
• Entrez Gene: SIZ1 (a.k.a. YDR409W, ULL1)
• Tags / Fusion Proteins:
  • T7 (N terminal on backbone)
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry