pKP920
(Plasmid
#160814)
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PurposeExpression of ExbB protein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 160814 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBAD24
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameexbB
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SpeciesEscherichia coli
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Extra-long PCR used to delete exbD gene, resulting in exbB-only
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKP920 was a gift from Kathleen Postle (Addgene plasmid # 160814 ; http://n2t.net/addgene:160814 ; RRID:Addgene_160814) -
For your References section:
The ExbD periplasmic domain contains distinct functional regions for two stages in TonB energization. Ollis AA, Kumar A, Postle K. J Bacteriol. 2012 Jun;194(12):3069-77. doi: 10.1128/JB.00015-12. Epub 2012 Apr 6. 10.1128/JB.00015-12 PubMed 22493019