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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16078 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti6/V5-GW/lacZ
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 10127
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesoluble adenylyl cyclase
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Alt namesAC
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SpeciesH. sapiens (human)
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Entrez GeneADCY10 (a.k.a. HCA2, HEL-S-7a, SAC, SACI, Sacy, hsAC)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site attB1 (destroyed during cloning)
- 3′ cloning site attB2 (destroyed during cloning)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti6/V5 human sAC was a gift from Jochen Buck & Lonny Levin (Addgene plasmid # 16078)