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pcDNA3.1 5'-myr-dEGFP-3'
(Plasmid #16075)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16075 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Protein synthesis reporter
  • Alt name
    5'UTR-myr-dEGFP-3'UTR
  • Alt name
    CaMKII alpha
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4400
  • Entrez Gene
    Camk2a (a.k.a. CaMKII, mKIAA0968)
  • Tag / Fusion Protein
    • EGFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site See map (not destroyed)
  • 3′ cloning site See map (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The coding sequence of green fluorescent protein is flanked by the 5' and 3' untranslated regions from CAMKII-alpha, conferring both dendritic mRNA localization and translational regulation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 5'-myr-dEGFP-3' was a gift from Erin Schuman (Addgene plasmid # 16075 ; http://n2t.net/addgene:16075 ; RRID:Addgene_16075)

  • For your References section:

    Dynamic visualization of local protein synthesis in hippocampal neurons. Aakalu G, Smith WB, Nguyen N, Jiang C, Schuman EM. Neuron. 2001 May . 30(2):489-502. 10.1016/S0896-6273(01)00295-1 PubMed 11395009
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