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Addgene

pcDNA3.1 CIM5 (Rpt-1) GFP
(Plasmid #16074)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16074 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CIM5
  • Alt name
    Rpt-1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1400
  • Entrez Gene
    RPT1 (a.k.a. YKL145W, CIM5, YTA3)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 CIM5 (Rpt-1) GFP was a gift from Erin Schuman (Addgene plasmid # 16074 ; http://n2t.net/addgene:16074 ; RRID:Addgene_16074)

  • For your References section:

    Activity-dependent dynamics and sequestration of proteasomes in dendritic spines. Bingol B, Schuman EM. Nature. 2006 Jun 29. 441(7097):1144-8. 10.1038/nature04769 PubMed 16810255
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