pEGFP-C1 beta-catenin Y654E
(Plasmid #16073)

• Depositing Lab: Erin Schuman
• Publication: Murase et al Neuron. 2002 Jul 3. 35(1):91-105.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: beta-catenin
• Species: G. gallus (chicken)
• Insert Size (bp): 2345
• Mutation: Y654E, *(see below)
• Entrez Gene: CTNNB1 (a.k.a. CHBCAT)
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BspE1/BamHI (destroyed during cloning)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Phosphorylation-mimic mutant of beta-catenin.

*Note: In addition to the described mutation, Addgene's quality control sequencing finds the following polymorphisms: L95R, S96A, G636A, and V651G.