-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 16072 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepEGFP-C1
-
Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namebeta-catenin
-
SpeciesG. gallus (chicken)
-
Insert Size (bp)2345
-
MutationY654F, *(see below)
-
Entrez GeneCTNNB1 (a.k.a. CHBCAT)
-
Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BspE1/BamHI (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Phosphorylation-prevented mutant of beta catenin.
*Note: In addition to the described mutation, Addgene's quality control sequencing finds the following polymorphisms: L95R, S96A, G636A, and V651G.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pEGFP-C1 beta-catenin Y654F was a gift from Erin Schuman (Addgene plasmid # 16072 ; http://n2t.net/addgene:16072 ; RRID:Addgene_16072) -
For your References section:
Depolarization drives beta-Catenin into neuronal spines promoting changes in synaptic structure and function. Murase S, Mosser E, Schuman EM. Neuron. 2002 Jul 3. 35(1):91-105. 10.1016/S0896-6273(02)00764-X PubMed 12123611