pTrichoGate-10
(Plasmid
#160710)
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PurposeFor C-terminal tags with Trichoderma optimised Synthetic mCherry bordered by BsaI restriction sites
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 160710 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepICH41021
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTrichoderma optimised Synthetic mCherry
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SpeciesSynthetic
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GenBank IDMN007114
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTrichoGate-10 was a gift from Artemio Mendoza (Addgene plasmid # 160710 ; http://n2t.net/addgene:160710 ; RRID:Addgene_160710) -
For your References section:
TrichoGate: An Improved Vector System for a Large Scale of Functional Analysis of Trichoderma Genes. Nogueira-Lopez G, Padilla-Arizmendi F, Inwood S, Lyne S, Steyaert JM, Nieto-Jacobo MF, Stewart A, Mendoza-Mendoza A. Front Microbiol. 2019 Dec 10;10:2794. doi: 10.3389/fmicb.2019.02794. eCollection 2019. 10.3389/fmicb.2019.02794 PubMed 31921006
