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Addgene

pCDNA3-FLAG-human NPM1-C1
(Plasmid #160693)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160693 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NPM1
  • Species
    H. sapiens (human)
  • Mutation
    Lacks NBD domain
  • Entrez Gene
    NPM1 (a.k.a. B23, NPM)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3-FLAG-human NPM1-C1 was a gift from Charles Sherr (Addgene plasmid # 160693 ; http://n2t.net/addgene:160693 ; RRID:Addgene_160693)

  • For your References section:

    Physical and functional interactions of the Arf tumor suppressor protein with nucleophosmin/B23. Bertwistle D, Sugimoto M, Sherr CJ. Mol Cell Biol. 2004 Feb . 24(3):985-96. 10.1128/MCB.24.3.985-996.2004 PubMed 14729947
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