pGEX-2T-RNAPII-A2-14-HA
(Plasmid #160689)

• Purpose: Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains an S-to-A mutation in position 2 of 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.
• Depositing Lab: Jeffry Corden
• Publication: Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94. doi: 10.1074/jbc.273.8.4689.

Backbone

• Vector backbone: pGEX-2T
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RNA polymerase II CTD
• Species: S. cerevisiae (budding yeast)
• Mutation: Contains an S-to-A mutation in position 2 of 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pGEX-2T -RNAP II CTD A214-HA.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry