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Addgene

pGEX-2T-RNAPII-CTD wt #14-HA
(Plasmid #160688)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160688 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEX-2T
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RNA Polymerase II CTD
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    C terminal domain only

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-2T-RNAPII-CTD wt #14-HA was a gift from Jeffry Corden (Addgene plasmid # 160688 ; http://n2t.net/addgene:160688 ; RRID:Addgene_160688)
  • For your References section:

    Growth-related changes in phosphorylation of yeast RNA polymerase II. Patturajan M, Schulte RJ, Sefton BM, Berezney R, Vincent M, Bensaude O, Warren SL, Corden JL. J Biol Chem. 1998 Feb 20;273(8):4689-94. doi: 10.1074/jbc.273.8.4689. 10.1074/jbc.273.8.4689 PubMed 9468530