pGEX-2T-RNAPII-CTD wt #14-HA
(Plasmid
#160688)
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PurposeExpression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 160688 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-2T
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRNA Polymerase II CTD
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SpeciesS. cerevisiae (budding yeast)
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MutationC terminal domain only
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-2T-RNAPII-CTD wt #14-HA was a gift from Jeffry Corden (Addgene plasmid # 160688 ; http://n2t.net/addgene:160688 ; RRID:Addgene_160688) -
For your References section:
Growth-related changes in phosphorylation of yeast RNA polymerase II. Patturajan M, Schulte RJ, Sefton BM, Berezney R, Vincent M, Bensaude O, Warren SL, Corden JL. J Biol Chem. 1998 Feb 20;273(8):4689-94. doi: 10.1074/jbc.273.8.4689. 10.1074/jbc.273.8.4689 PubMed 9468530