pEG BacMam C term 3C eGFP myc
(Plasmid
#160685)
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Purpose(Empty Backbone) Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 160685 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEG BacMam
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Modifications to backbonepEG BacMam C terminus tagged with 3C eGFP myc
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Vector typeMammalian Expression
- Promoter CMV
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Tag
/ Fusion Protein
- His8 eGFP 3C (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEG BacMam C term 3C eGFP myc was a gift from Eric Gouaux (Addgene plasmid # 160685 ; http://n2t.net/addgene:160685 ; RRID:Addgene_160685) -
For your References section:
Screening and large-scale expression of membrane proteins in mammalian cells for structural studies. Goehring A, Lee CH, Wang KH, Michel JC, Claxton DP, Baconguis I, Althoff T, Fischer S, Garcia KC, Gouaux E. Nat Protoc. 2014 Nov;9(11):2574-85. doi: 10.1038/nprot.2014.173. Epub 2014 Oct 9. 10.1038/nprot.2014.173 PubMed 25299155
Map uploaded by the depositor.
