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Addgene

pJMP2836
(Plasmid #160674)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160674 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    R6Kγ
  • Vector type
    Bacterial Expression
  • Selectable markers
    Spectinomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BW25141
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gmc6 (gfp) sgRNA
  • gRNA/shRNA sequence
    CATCTAATTCAACAAGAATT
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJMP2836 was a gift from Jason Peters (Addgene plasmid # 160674 ; http://n2t.net/addgene:160674 ; RRID:Addgene_160674)

  • For your References section:

    Programmable Gene Knockdown in Diverse Bacteria Using Mobile-CRISPRi. Banta AB, Ward RD, Tran JS, Bacon EE, Peters JM. Curr Protoc Microbiol. 2020 Dec;59(1):e130. doi: 10.1002/cpmc.130. 10.1002/cpmc.130 PubMed 33332762
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