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PurposeExpresses GFP fusion of a multisite mutant of the Af1521 protein
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 160665 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepNIC-28
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Backbone manufacturerStructural Genomics Gonsortium
- Total vector size (bp) 6675
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameAf1521 modified synthetic construct eGFP fusion
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Alt name"MacroGreen"
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SpeciesSynthetic; Archaeoglobus fulgidus
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Insert Size (bp)1266
- Promoter LacI
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Tags
/ Fusion Proteins
- hexahistidine_TEV recognition (N terminal on backbone)
- eGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNIC28-MacroGreen was a gift from Herwig Schüler (Addgene plasmid # 160665 ; http://n2t.net/addgene:160665 ; RRID:Addgene_160665) -
For your References section:
MacroGreen, a simple tool for detection of ADP-ribosylated proteins. Garcia-Saura AG, Herzog LK, Dantuma NP, Schuler H. Commun Biol. 2021 Jul 28;4(1):919. doi: 10.1038/s42003-021-02439-w. 10.1038/s42003-021-02439-w PubMed 34321589
Map uploaded by the depositor.
