10TALE_Pmin_fLuc
(Plasmid #160663)

• Purpose: Expression of the firefly luciferase reporter upon binding of a transcriptional activator to target sites. The plasmid contains 10 copies of target sites for the TALEN1257 DNA-binding domain
• Depositing Lab: Roman Jerala
• Publication: Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-2065. doi: 10.1021/acssynbio.0c00133. Epub 2020 Jul 20.

Backbone

• Vector backbone: pGL4.16
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 10TALE_Pmin_fLuc
• Species: Synthetic
• Promoter: minimal

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GTGTGAATCGATAGTACTAACATAC
• 3′ sequencing primer: cactgcattctagttgtggtttgtcc

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gaber, R., Lebar, T., Majerle, A. et al. Designable DNA-binding domains enable construction of logic circuits in mammalian cells. Nat Chem Biol 10, 203–208 (2014). https://doi.org/10.1038/nchembio.1433

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry