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Addgene

pcI-ts,ind+-(NSP8)
(Plasmid #160656)

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Item Catalog # Description Quantity Price (USD)
Plasmid 160656 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pcI-ts,ind+
  • Backbone size w/o insert (bp) 3482
  • Total vector size (bp) 4082
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Growth at 37C or addition of nalidixic acid induces NSP8 expression
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 NSP8
  • Alt name
    Severe acute respiratory syndrome coronavirus 2 non-structural protein 8
  • Species
    Synthetic
  • Insert Size (bp)
    600
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Codon optimized NSP8 gene was ordered from Genscript.
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcI-ts,ind+-(NSP8) was a gift from Kenneth Johnson (Addgene plasmid # 160656 ; http://n2t.net/addgene:160656 ; RRID:Addgene_160656)

  • For your References section:

    Remdesivir Is Effective in Combating COVID-19 because It Is a Better Substrate than ATP for the Viral RNA-Dependent RNA Polymerase. Dangerfield TL, Huang NZ, Johnson KA. iScience. 2020 Nov 28:101849. doi: 10.1016/j.isci.2020.101849. 10.1016/j.isci.2020.101849 PubMed 33283177
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