ClCry4 Y319D
(Plasmid #160649)

• Purpose: Expression of protein for various experiments including finding interaction partners
• Depositing Lab: Joseph Takahashi
• Publication: Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;116(39):19449-19457. doi: 10.1073/pnas.1907875116. Epub 2019 Sep 4.

Backbone

• Vector backbone: pfastBac HTb
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 4856
• Total vector size (bp): 6400
• Vector type: Bacterial Expression
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Clcry4 PHR Y319D
• Alt name: Pigeon Cry4 PHR Y319D
• Species: C.livia
• Insert Size (bp): 1491
• Tag / Fusion Protein:
  • Hexa his (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: pfastbac forward
• 3′ sequencing primer: pfastbac reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry