Skip to main content
Addgene

pDGB3 omega1 Tnos:nptII:Pnos - P35S:Cas9:Tnos - P35S:DsRed:Tnos (GB2235)
(Plasmid #160646)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160646 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEGB3_omega1
  • Backbone manufacturer
    self-made; derived from pCambia1302 generated at the Cambia Institute
  • Vector type
    CRISPR
  • Selectable markers
    lacZ

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tNos:nptII:PNos-P35s:Cas9:tNos-P35s:DsRed:tNos
  • Species
    Unspecified
  • Insert Size (bp)
    9357
  • Mutation
    BsaI and BsmBI sites removed
  • Promoter Pnos, 35S, 35S

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)
  • 5′ sequencing primer caacctctcgggcttctgga
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Compatible with GoldenBraid; insert can be released with BsaI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDGB3 omega1 Tnos:nptII:Pnos - P35S:Cas9:Tnos - P35S:DsRed:Tnos (GB2235) was a gift from Diego Orzaez (Addgene plasmid # 160646 ; http://n2t.net/addgene:160646 ; RRID:Addgene_160646)

  • For your References section:

    The GB4.0 Platform, an All-In-One Tool for CRISPR/Cas-Based Multiplex Genome Engineering in Plants. Vazquez-Vilar M, Garcia-Carpintero V, Selma S, Bernabe-Orts JM, Sanchez-Vicente J, Salazar-Sarasua B, Ressa A, de Paola C, Ajenjo M, Quintela JC, Fernandez-Del-Carmen A, Granell A, Orzaez D. Front Plant Sci. 2021 Jul 1;12:689937. doi: 10.3389/fpls.2021.689937. eCollection 2021. 10.3389/fpls.2021.689937 PubMed 34276739
Related items:
Commonly requested with: