pUPD2 SV40-AcrIIA4 (GB3333)
(Plasmid
#160605)
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PurposeSV40-AcrIIA4 codon optimized for N.benthamiana
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 160605 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUPD2
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Backbone manufacturerself-made; derived from the BioBrick assembly plasmid pSB1C3
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAcrIIA4
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SpeciesSynthetic
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Insert Size (bp)290
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MutationBsaI and BsmBI sites removed
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsmBI (destroyed during cloning)
- 3′ cloning site BsmBI (destroyed during cloning)
- 5′ sequencing primer gctttcgctaaggatgatttctgg
- 3′ sequencing primer cagggtggtgacaccttgcc (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Compatible with GoldenBraid; insert can be released with BsaI
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUPD2 SV40-AcrIIA4 (GB3333) was a gift from Diego Orzaez (Addgene plasmid # 160605 ; http://n2t.net/addgene:160605 ; RRID:Addgene_160605) -
For your References section:
Strong and tunable anti-CRISPR/Cas activities in plants. Calvache C, Vazquez-Vilar M, Selma S, Uranga M, Fernandez-Del-Carmen A, Daros JA, Orzaez D. Plant Biotechnol J. 2022 Feb;20(2):399-408. doi: 10.1111/pbi.13723. Epub 2021 Oct 31. 10.1111/pbi.13723 PubMed 34632687