pcDNA3 mId1
(Plasmid #16060)

• Depositing Lab: Robert Benezra
• Publication: Id1 plasmids (unpublished)

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Id1
• Species: M. musculus (mouse)
• Insert Size (bp): 500
• Entrez Gene: Id1 (a.k.a. AI323524, D2Wsu140e, Idb1, bHLHb24)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Supplemental Documents:
  • pcDNA3-mId1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

mId1 protein coding sequence was amplified from a lab stock plasmid by high fidelity PCR. EcoRI and BamHI restriction sites were added to the 5' and 3' ends, respectively. This fragment was first cloned into EcoRI and BamHI sites of pBluescript II KS (-), then the HindIII-NotI fragment from that plasmid was subcloned into HindIII and NotI sites of pcDNA3.