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Addgene

pVD1 tRNA-gRNA E4-En-1 (GB2243)
(Plasmid #160565)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160565 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pVD1
  • Backbone manufacturer
    self-made; derived from pGEMT-Easy manufactured by Promega
  • Vector type
    CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tRNA-gRNA position E4-En-1 (Multiplexing Edit)
  • Species
    Synthetic
  • Insert Size (bp)
    189
  • Mutation
    BsaI and BsmBI sites removed

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Compatible with GoldenBraid; insert can be released with BsmBI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVD1 tRNA-gRNA E4-En-1 (GB2243) was a gift from Diego Orzaez (Addgene plasmid # 160565 ; http://n2t.net/addgene:160565 ; RRID:Addgene_160565)

  • For your References section:

    The GB4.0 Platform, an All-In-One Tool for CRISPR/Cas-Based Multiplex Genome Engineering in Plants. Vazquez-Vilar M, Garcia-Carpintero V, Selma S, Bernabe-Orts JM, Sanchez-Vicente J, Salazar-Sarasua B, Ressa A, de Paola C, Ajenjo M, Quintela JC, Fernandez-Del-Carmen A, Granell A, Orzaez D. Front Plant Sci. 2021 Jul 1;12:689937. doi: 10.3389/fpls.2021.689937. eCollection 2021. 10.3389/fpls.2021.689937 PubMed 34276739
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