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Addgene

5' Del 8
(Plasmid #16056)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16056 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL2-basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5600
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Id1 promoter, 5' deletion
  • Alt name
    Id1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    140
  • Mutation
    -52 to +88 of promoter
  • Entrez Gene
    Id1 (a.k.a. AI323524, D2Wsu140e, Idb1, bHLHb24)
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer na
  • 3′ sequencing primer LucNrev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    5' Del 8 was a gift from Robert Benezra (Addgene plasmid # 16056 ; http://n2t.net/addgene:16056 ; RRID:Addgene_16056)

  • For your References section:

    Transcription of the dominant-negative helix-loop-helix protein Id1 is regulated by a protein complex containing the immediate-early response gene Egr-1. Tournay O, Benezra R. Mol Cell Biol. 1996 May . 16(5):2418-30. PubMed 8628310
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