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pUPD P35S (GB0552)
(Plasmid #160554)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160554 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUPD
  • Backbone manufacturer
    self-made; derived from pGEMT-Easy manufactured by Promega
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    35s
  • Species
    Cauliflower mosaic virus
  • Insert Size (bp)
    291
  • Mutation
    BsaI and BsmBI sites removed

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Compatible with GoldenBraid; insert can be released with BsaI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUPD P35S (GB0552) was a gift from Diego Orzaez (Addgene plasmid # 160554 ; http://n2t.net/addgene:160554 ; RRID:Addgene_160554)

  • For your References section:

    GB3.0: a platform for plant bio-design that connects functional DNA elements with associated biological data. Vazquez-Vilar M, Quijano-Rubio A, Fernandez-Del-Carmen A, Sarrion-Perdigones A, Ochoa-Fernandez R, Ziarsolo P, Blanca J, Granell A, Orzaez D. Nucleic Acids Res. 2017 Feb 28;45(4):2196-2209. doi: 10.1093/nar/gkw1326. 10.1093/nar/gkw1326 PubMed 28053117
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