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Addgene

ClCry4 PHR
(Plasmid #160552)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160552 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Pfast HTb
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4856
  • Total vector size (bp) 6400
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Clcry4 HR
  • Alt name
    Pigeon Cry4
  • Species
    C.livia
  • Insert Size (bp)
    1491
  • Tag / Fusion Protein
    • Hexa his

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer pfastbac forward
  • 3′ sequencing primer pfastbac reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ClCry4 PHR was a gift from Joseph Takahashi (Addgene plasmid # 160552 ; http://n2t.net/addgene:160552 ; RRID:Addgene_160552)

  • For your References section:

    Chemical and structural analysis of a photoactive vertebrate cryptochrome from pigeon. Zoltowski BD, Chelliah Y, Wickramaratne A, Jarocha L, Karki N, Xu W, Mouritsen H, Hore PJ, Hibbs RE, Green CB, Takahashi JS. Proc Natl Acad Sci U S A. 2019 Sep 24;116(39):19449-19457. doi: 10.1073/pnas.1907875116. Epub 2019 Sep 4. 10.1073/pnas.1907875116 PubMed 31484780
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