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Addgene

pLenti-CMV-MARCer-Blast
(Plasmid #160550)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160550 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
  • Backbone size w/o insert (bp) 7592
  • Vector type
    Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MARCer
  • Alt name
    insert encodes amino acids 1-603 of the human HIF-1α protein fused to eGFP-CreERT2
  • Species
    H. sapiens (human)
  • Entrez Gene
    HIF1A (a.k.a. HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)
  • Promoter CMV
  • Tag / Fusion Protein
    • eGFP-Cre (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BstXI (not destroyed)
  • 3′ cloning site BstXI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-CMV-MARCer-Blast was a gift from Marc Vooijs (Addgene plasmid # 160550 ; http://n2t.net/addgene:160550 ; RRID:Addgene_160550)

  • For your References section:

    A lineage-tracing tool to map the fate of hypoxic tumour cells. Vermeer JAF, Ient J, Markelc B, Kaeppler J, Barbeau LMO, Groot AJ, Muschel RJ, Vooijs MA. Dis Model Mech. 2020 Jul 30;13(7). pii: dmm.044768. doi: 10.1242/dmm.044768. 10.1242/dmm.044768 PubMed 32571767
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