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Addgene

p_m16S
(Plasmid #160549)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160549 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBSK (+)
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    16S rDNA fragment from M. capricolum subsp. capricolum strain California Kid (gi_83283139)
  • Alt name
    m16S
  • Insert Size (bp)
    913
  • GenBank ID
    gi_83283139

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR V (destroyed during cloning)
  • 3′ cloning site EcoR V (destroyed during cloning)
  • 5′ sequencing primer -
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p_m16S was a gift from Denis Gerlier (Addgene plasmid # 160549 ; http://n2t.net/addgene:160549 ; RRID:Addgene_160549)

  • For your References section:

    Assessing mycoplasma contamination of cell cultures by qPCR using a set of universal primer pairs targeting a 1.5 kb fragment of 16S rRNA genes. Jean A, Tardy F, Allatif O, Grosjean I, Blanquier B, Gerlier D. PLoS One. 2017 Feb 22;12(2):e0172358. doi: 10.1371/journal.pone.0172358. eCollection 2017. 10.1371/journal.pone.0172358 PubMed 28225826
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