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Addgene

pEFEX1-A2aGH
(Plasmid #160543)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160543 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYES2
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.
  • Species
    H. sapiens (human), S. cerevisiae (budding yeast)
  • Mutation
    N/A
  • Entrez Gene
    FEX1 (a.k.a. YOR390W)
  • Entrez Gene
    ADORA2A (a.k.a. A2aR, ADORA2, RDC8)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEFEX1-A2aGH was a gift from Michelle O'Malley (Addgene plasmid # 160543 ; http://n2t.net/addgene:160543 ; RRID:Addgene_160543)

  • For your References section:

    Engineered fluoride sensitivity enables biocontainment and selection of genetically-modified yeasts. Yoo JI, Seppala S, O'Malley MA. Nat Commun. 2020 Oct 29;11(1):5459. doi: 10.1038/s41467-020-19271-1. 10.1038/s41467-020-19271-1 PubMed 33122649
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