pQE-(NSP12)-pcI-ts,ind+-(NSP7-NSP8)
(Plasmid #160540)

• Purpose: Expresses codon optimized SARS-CoV-2 NSP7, NSP8, and NSP12 in E. coli.
• Depositing Lab: Kenneth Johnson
• Publication: Dangerfield et al iScience. 2020 Nov 28:101849. doi: 10.1016/j.isci.2020.101849.

Backbone

• Vector backbone: pQE-30 and pcI-ts,ind+
• Total vector size (bp): 9813
• Modifications to backbone: CmR gene from pQE-30 removed and AmpR from pcI-ts,ind+ removed.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Growth instructions: Growth at 37 or addition of nalidixic acid induces expression of NSP7 and NSP8
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: SARS-CoV-2 NSP7
• Alt name: Severe acute respiratory syndrome coronavirus 2 non-structural protein 7
• Species: Synthetic
• Insert Size (bp): 255
• Entrez Gene: ORF1ab (a.k.a. GU280_gp01)

Gene/Insert 2

• Gene/Insert name: SARS-CoV-2 NSP8
• Alt name: Severe acute respiratory syndrome coronavirus 2 non-structural protein 8
• Species: Synthetic
• Insert Size (bp): 600
• Entrez Gene: ORF1ab (a.k.a. GU280_gp01)

Gene/Insert 3

• Gene/Insert name: SARS-CoV-2 NSP12
• Alt name: Severe acute respiratory syndrome coronavirus 2 non-structural protein 12
• Species: Synthetic
• Entrez Gene: ORF1ab (a.k.a. GU280_gp01)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Codon optimized NSP7, NSP8, and NSP12 genes were from custom plasmids synthesized by Genscript.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA