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Addgene

pTricHis-PLY-WT-charge+
(Plasmid #160538)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160538 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTricHisA
  • Backbone manufacturer
    ThermoFisher
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Depositor recommends using BL21 (DE3) for protein expression and purification.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PLY-WT-charge+
  • Species
    Other
  • Insert Size (bp)
    1524
  • Mutation
    W278K, L282K
  • Tag / Fusion Protein
    • His-Tag (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ILY gene is codon optimized, and all native Cys removed, before additional mutations were added.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTricHis-PLY-WT-charge+ was a gift from Doryen Bubeck (Addgene plasmid # 160538 ; http://n2t.net/addgene:160538 ; RRID:Addgene_160538)

  • For your References section:

    Structural basis for tuning activity and membrane specificity of bacterial cytolysins. Shah NR, Voisin TB, Parsons ES, Boyd CM, Hoogenboom BW, Bubeck D. Nat Commun. 2020 Nov 16;11(1):5818. doi: 10.1038/s41467-020-19482-6. 10.1038/s41467-020-19482-6 PubMed 33199689
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