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Addgene

pCDH-CAG-NCreIntN-T2A-mTagBFP2
(Plasmid #160500)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160500 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH
  • Backbone manufacturer
    System Biosciences
  • Backbone size w/o insert (bp) 8550
  • Total vector size (bp) 9107
  • Vector type
    Mammalian Expression, Lentiviral, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NCreIntN
  • Species
    Synthetic
  • Insert Size (bp)
    555
  • Promoter CAG
  • Tag / Fusion Protein
    • mTagBFP2

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer cggatctcgacggtatcggt
  • 3′ sequencing primer tcatcactcgttgcatcgac
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH-CAG-NCreIntN-T2A-mTagBFP2 was a gift from Shawn Je (Addgene plasmid # 160500 ; http://n2t.net/addgene:160500 ; RRID:Addgene_160500)

  • For your References section:

    Neural circuit analysis using a novel intersectional split intein-mediated split-Cre recombinase system. Khoo ATT, Kim PJ, Kim HM, Je HS. Mol Brain. 2020 Jul 2;13(1):101. doi: 10.1186/s13041-020-00640-2. 10.1186/s13041-020-00640-2 PubMed 32616061
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