hP2X3-cALFA
(Plasmid #160499)

• Purpose: For the expression of human P2X3 with ALFA tag at C-terminus in mammalian cells
• Depositing Lab: Motoyuki Hattori
• Publication: Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi: 10.1038/s42003-021-01891-y.

Backbone

• Vector backbone: pBMNb-cALFA
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human P2X3
• Species: H. sapiens (human), Synthetic
• Mutation: ΔN5ΔC33-T13P/S15V/V16I
• Entrez Gene: P2RX3 (a.k.a. P2X3)
• Tag / Fusion Protein:
  • HRV3C protease cleavage site-ALFA tag-twin Strep tag-8histidine (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: SalI (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry