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pVRC8400-SARS-CoV-2-RBD-L455RG496R-AVI
(Plasmid #160479)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160479 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pVRC8400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2-RBD-L455RG496R
  • Mutation
    changed Leucine 455 to Arginine and changed Glycine 496 to Arginine
  • Tags / Fusion Proteins
    • scFc (N terminal on insert)
    • HRV3C (N terminal on insert)
    • AVI (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVRC8400-SARS-CoV-2-RBD-L455RG496R-AVI was a gift from Peter Kwong (Addgene plasmid # 160479 ; http://n2t.net/addgene:160479 ; RRID:Addgene_160479)

  • For your References section:

    Structure-Based Design with Tag-Based Purification and In-Process Biotinylation Enable Streamlined Development of SARS-CoV-2 Spike Molecular Probes. Zhou T, Teng IT, Olia AS, Cerutti G, Gorman J, Nazzari A, Shi W, Tsybovsky Y, Wang L, Wang S, Zhang B, Zhang Y, Katsamba PS, Petrova Y, Banach BB, Fahad AS, Liu L, Lopez Acevedo SN, Madan B, Oliveira de Souza M, Pan X, Wang P, Wolfe JR, Yin M, Ho DD, Phung E, DiPiazza A, Chang LA, Abiona OM, Corbett KS, DeKosky BJ, Graham BS, Mascola JR, Misasi J, Ruckwardt T, Sullivan NJ, Shapiro L, Kwong PD. Cell Rep. 2020 Oct 27;33(4):108322. doi: 10.1016/j.celrep.2020.108322. Epub 2020 Oct 12. 10.1016/j.celrep.2020.108322 PubMed 33091382
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