Skip to main content
Addgene

VL3 (Flag-Mad2)
(Plasmid #16047)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16047 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pFlag-CMV2
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mad2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    600
  • Entrez Gene
    MAD2L1 (a.k.a. HSMAD2, MAD2)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VL3 (Flag-Mad2) was a gift from Robert Benezra (Addgene plasmid # 16047 ; http://n2t.net/addgene:16047 ; RRID:Addgene_16047)

  • For your References section:

    Mad2 phosphorylation regulates its association with Mad1 and the APC/C. Wassmann K, Liberal V, Benezra R. EMBO J. 2003 Feb 17. 22(4):797-806. 10.1093/emboj/cdg071 PubMed 12574116
Related items:
Commonly requested with: