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pHX_gRNA_hPAX7_6-9S
(Plasmid #160453)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160453 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    gRNA_Cloning Vector
  • Backbone manufacturer
    41824
  • Backbone size w/o insert (bp) 3910
  • Total vector size (bp) 3970
  • Modifications to backbone
    The AflII site was destroyed during cloning which results in a loss of 4 bp from the original backbone (3914 bp)
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gRNA_hPAX7_6-9S
  • gRNA/shRNA sequence
    GTGAGTGGGTGTACGTG
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_001135254
  • Promoter U6

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHX_gRNA_hPAX7_6-9S was a gift from April Pyle (Addgene plasmid # 160453 ; http://n2t.net/addgene:160453 ; RRID:Addgene_160453)

  • For your References section:

    Generation of PAX7 Reporter Cells to Investigate Skeletal Myogenesis from Human Pluripotent Stem Cells. Xi H, Young CS, Pyle AD. STAR Protoc. 2020 Nov 5;1(3):100158. doi: 10.1016/j.xpro.2020.100158. eCollection 2020 Dec 18. 10.1016/j.xpro.2020.100158 PubMed 33377052
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