pSB1C3-J23110-B0034-mRFP1_Orange
(Plasmid
#160444)
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PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Orange chromoprotein in E. coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 160444 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneBioBrick pSB1C3 vector
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Backbone manufactureriGEM Registry
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Modifications to backboneBioBrick sites removed
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepromoter, RBS, mRFP1E_Orange
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SpeciesSynthetic
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Insert Size (bp)810
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MutationBioBrick sites removed
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GenBank ID
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSB1C3-J23110-B0034-mRFP1_Orange was a gift from Anthony Forster (Addgene plasmid # 160444 ; http://n2t.net/addgene:160444 ; RRID:Addgene_160444) -
For your References section:
Overcoming chromoprotein limitations by engineering a red fluorescent protein. Bao L, Menon PNK, Liljeruhm J, Forster AC. Anal Biochem. 2020 Sep 3:113936. doi: 10.1016/j.ab.2020.113936. 10.1016/j.ab.2020.113936 PubMed 32891596