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pUC57-attB2-SA(2)-CRY2-mCherry
(Plasmid #160441)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160441 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Total vector size (bp) 9133
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cryptochrome 2
  • Species
    A. thaliana (mustard weed)
  • Entrez Gene
    CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC57-attB2-SA(2)-CRY2-mCherry was a gift from Nicholas Tolwinski (Addgene plasmid # 160441 ; http://n2t.net/addgene:160441 ; RRID:Addgene_160441)

  • For your References section:

    An Optogenetic Method to Study Signal Transduction in Intestinal Stem Cell Homeostasis. Bunnag N, Tan QH, Kaur P, Ramamoorthy A, Sung ICH, Lusk J, Tolwinski NS. J Mol Biol. 2020 May 1;432(10):3159-3176. doi: 10.1016/j.jmb.2020.03.019. Epub 2020 Mar 20. 10.1016/j.jmb.2020.03.019 PubMed 32201167
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