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Addgene

pBS/KS-mId2sp
(Plasmid #16044)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16044 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript KS-
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Id2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    195
  • Mutation
    cDNA nucleotides 1-195
  • Entrez Gene
    Id2 (a.k.a. Idb2, bHLHb26)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS/KS-mId2sp was a gift from Robert Benezra (Addgene plasmid # 16044 ; http://n2t.net/addgene:16044 ; RRID:Addgene_16044)

  • For your References section:

    Expression patterns of Id1, Id2, and Id3 are highly related but distinct from that of Id4 during mouse embryogenesis. Jen Y, Manova K, Benezra R. Dev Dyn. 1996 Nov . 207(3):235-52. 10.1002/(SICI)1097-0177(199611)207:3<235::AID-AJA1>3.0.CO;2-I PubMed 8922523
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