pUCIDT-attL1-Worm ABeta-attR5
(Plasmid #160437)

• Purpose: Entry vector for cloning nematode-codon-optimized Amyloid Beta(1-42) using 2-fragment gateway recombination
• Depositing Lab: Nicholas Tolwinski
• Publication: Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi: 10.21769/BioProtoc.3856. eCollection 2020 Dec 5.

Backbone

• Vector backbone: pUCIDT
• Total vector size (bp): 3118
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Homo sapiens amyloid beta precursor protein
• Species: H. sapiens (human), C. elegans (nematode)
• Mutation: Codon-optimized for expression in C. elegans
• Entrez Gene: APP (a.k.a. AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4)
• Tag / Fusion Protein:
  • Cleavage signal (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13 For
• 3′ sequencing primer: M13 Rev

Resource Information

• Supplemental Documents:
  • pUCIDT-attL1-Worm ABeta-attR5.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry