pBS/KS-mId1sp
(Plasmid
#16043)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16043 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript KS-
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameId1
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SpeciesM. musculus (mouse)
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Insert Size (bp)350
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MutationcDNA nucleotides 390-742
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Entrez GeneId1 (a.k.a. AI323524, D2Wsu140e, Idb1, bHLHb24)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS/KS-mId1sp was a gift from Robert Benezra (Addgene plasmid # 16043 ; http://n2t.net/addgene:16043 ; RRID:Addgene_16043) -
For your References section:
Expression patterns of Id1, Id2, and Id3 are highly related but distinct from that of Id4 during mouse embryogenesis. Jen Y, Manova K, Benezra R. Dev Dyn. 1996 Nov . 207(3):235-52. 10.1002/(SICI)1097-0177(199611)207:3<235::AID-AJA1>3.0.CO;2-I PubMed 8922523