pAAV-CaMKII-iGECI
(Plasmid
#160423)
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PurposeAAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 160423 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-CW3SL
- Backbone size w/o insert (bp) 3755
- Total vector size (bp) 6125
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameiGECI
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SpeciesSynthetic
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Insert Size (bp)2394
- Promoter CaMKII
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer gagtggcccctagttctg
- 3′ sequencing primer tttgtaatccagaggttg (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-CaMKII-iGECI was a gift from Vladislav Verkhusha (Addgene plasmid # 160423 ; http://n2t.net/addgene:160423 ; RRID:Addgene_160423) -
For your References section:
A near-infrared genetically encoded calcium indicator for in vivo imaging. Shemetov AA, Monakhov MV, Zhang Q, Canton-Josh JE, Kumar M, Chen M, Matlashov ME, Li X, Yang W, Nie L, Shcherbakova DM, Kozorovitskiy Y, Yao J, Ji N, Verkhusha VV. Nat Biotechnol. 2021 Mar;39(3):368-377. doi: 10.1038/s41587-020-0710-1. Epub 2020 Oct 26. 10.1038/s41587-020-0710-1 PubMed 33106681