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Addgene

piGECI-NES-N1
(Plasmid #160422)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160422 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pN1
  • Backbone size w/o insert (bp) 3939
  • Total vector size (bp) 6378
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iGECI-NES
  • Species
    Synthetic
  • Insert Size (bp)
    2439
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer agagctggtttagtgaac
  • 3′ sequencing primer gtggtatggctgattatg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    piGECI-NES-N1 was a gift from Vladislav Verkhusha (Addgene plasmid # 160422 ; http://n2t.net/addgene:160422 ; RRID:Addgene_160422)

  • For your References section:

    A near-infrared genetically encoded calcium indicator for in vivo imaging. Shemetov AA, Monakhov MV, Zhang Q, Canton-Josh JE, Kumar M, Chen M, Matlashov ME, Li X, Yang W, Nie L, Shcherbakova DM, Kozorovitskiy Y, Yao J, Ji N, Verkhusha VV. Nat Biotechnol. 2021 Mar;39(3):368-377. doi: 10.1038/s41587-020-0710-1. Epub 2020 Oct 26. 10.1038/s41587-020-0710-1 PubMed 33106681
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