pRK5 beta1
(Plasmid #16042)

• Depositing Lab: Filippo Giancotti
• Publication: Giancotti et al Cell. 1990 Mar 9. 60(5):849-59.

Backbone

• Vector backbone: pRK5
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: beta1
• Alt name: beta 1 integrin
• Species: H. sapiens (human)
• Insert Size (bp): 3600
• Entrez Gene: ITGB1 (a.k.a. CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the beta-1 integrin ORF has the following amino acid changes - C17G, M170T and N227S. Addgene is not aware of the impact of these mutations on the plasmid function.