pJH198_pE12_hyCal2-eGFP
(Plasmid
#160411)
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PurposeGateway entry clone containing postsynaptic HyCal component (HyNaC2) for HySyn system fused to eGFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 160411 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221
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Vector typeOther
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepDONR221 HyCal2::eGFP
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Alt namehyNaC2
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SpeciesSynthetic
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Tag
/ Fusion Protein
- eGFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bySynthesis
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJH198_pE12_hyCal2-eGFP was a gift from Daniel Colón-Ramos (Addgene plasmid # 160411 ; http://n2t.net/addgene:160411 ; RRID:Addgene_160411) -
For your References section:
A genetically encoded tool for reconstituting synthetic modulatory neurotransmission and reconnect neural circuits in vivo. Hawk JD, Wisdom EM, Sengupta T, Kashlan ZD, Colon-Ramos DA. Nat Commun. 2021 Aug 9;12(1):4795. doi: 10.1038/s41467-021-24690-9. 10.1038/s41467-021-24690-9 PubMed 34373460