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Addgene

pJH198_pE12_hyCal2-eGFP
(Plasmid #160411)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160411 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDONR221
  • Vector type
    Other

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pDONR221 HyCal2::eGFP
  • Alt name
    hyNaC2
  • Species
    Synthetic
  • Tag / Fusion Protein
    • eGFP (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJH198_pE12_hyCal2-eGFP was a gift from Daniel Colón-Ramos (Addgene plasmid # 160411 ; http://n2t.net/addgene:160411 ; RRID:Addgene_160411)
  • For your References section:

    A genetically encoded tool for reconstituting synthetic modulatory neurotransmission and reconnect neural circuits in vivo. Hawk JD, Wisdom EM, Sengupta T, Kashlan ZD, Colon-Ramos DA. Nat Commun. 2021 Aug 9;12(1):4795. doi: 10.1038/s41467-021-24690-9. 10.1038/s41467-021-24690-9 PubMed 34373460