pcDNA3.1/Myc-His beta4
(Plasmid #16039)

• Depositing Lab: Filippo Giancotti
• Publication: Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-502.

Backbone

• Vector backbone: pcDNA3.1(-)/Myc-His B
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: beta4
• Alt name: beta 4 integrin
• Species: H. sapiens (human)
• Insert Size (bp): 5600
• Entrez Gene: ITGB4 (a.k.a. CD104, GP150, JEB5A, JEB5B)
• Tags / Fusion Proteins:
  • Myc (C terminal on backbone)
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: The plasmid contains a S1694T mutation compared to NCBI reference sequence AAC51632.1. This mutation is a natural variant which is not expected to affect plasmid function.