Skip to main content
Addgene

GFP-ATG8(416)/GFP-AUT7(416)
(Plasmid #160389)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160389 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pAG36
  • Backbone size w/o insert (bp) 5313
  • Total vector size (bp) 7936
  • Vector type
    Yeast Expression ; CEN/ARS
  • Selectable markers
    Nourseothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    autophagy-related 8
  • Alt name
    AUT7
  • Alt name
    ATG8
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1077
  • Entrez Gene
    ATG8 (a.k.a. YBL078C, APG8, AUT7, CVT5)
  • Promoter Endogenous ATG8 promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NsiI (not destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer NA
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Insert obtained from GFP-ATG8(416)/GFP-AUT7(416) (Addgene 49425)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-ATG8(416)/GFP-AUT7(416) was a gift from John McCusker (Addgene plasmid # 160389 ; http://n2t.net/addgene:160389 ; RRID:Addgene_160389)
Related items:
Commonly requested with: