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Addgene

pCAS-mak10 gRNA
(Plasmid #160385)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160385 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAS
  • Backbone manufacturer
    Addgene $ 60847
  • Backbone size w/o insert (bp) 8761
  • Total vector size (bp) 8743
  • Vector type
    Yeast Expression
  • Selectable markers
    G418

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mak10 gRNA
  • Alt name
    MAK10
  • Alt name
    YEL053C
  • gRNA/shRNA sequence
    TTTCAATTAGCTCATCCAGG
  • Species
    S. cerevisiae (budding yeast)
  • Promoter RNA pol III promoter (tRNA-Tyr)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAS-mak10 gRNA was a gift from John McCusker (Addgene plasmid # 160385 ; http://n2t.net/addgene:160385 ; RRID:Addgene_160385)
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